Fig. 3

In situ protein and mRNA expression analysis for Axl and Igf1 in Iba1-positive microglia. Immunofluorescence imaging for Axl and Iba1 in representative cortical A and spinal cord B sections from rNLS8 mice at the late disease stage (i.e., DOX withdrawn for 6 weeks). C In situ hybridization for Axl mRNA (red) with hTDP-43 immunohistochemical co-staining (brown) in an rNLS8 cortical section, showing Axl-expressing microglia (white arrow) proximal to sites of hTDP-43 deposition. In situ hybridization for Igf1 mRNA (red) with Iba1 immunohistochemical co-staining (brown) in cortical sections from wild type/non-transgenic D and rNLS8 mice E, where the white arrow marks Axl-expressing Iba1-positive microglia