Pcp2-tTA;TRE-hShh mice show PC-specific hShh overexpression and enhance Shh signaling in cerebellum. A X-gal staining of sagittal brain sections of TRE-LacZ and Pcp2-tTA;TRE-LacZ at P0, P6, and P14. Brain sections were counter-stained with nuclear fast red, and cerebellum was shown. B Scheme of generating B6.Pcp2-tTA;TRE-hShh double transgenic mice. C Representative tile scan confocal images of a sagittal brain section of a 2-month-old Pcp2-hShh mouse. GFP channel was shown. D Immunostaining of Pcp2-hShh sagittal brain sections for Calbindin (red). Granular layer (GL), PC, and molecular layer (ML) were shown, and see Additional file 3: Video S3 for details. E FACS of cerebellums from TRE-hShh, Camk2a-hShh, and Pcp2-hShh mice. F Taqman RT-PCR for hShh, mGli1, and mPTCH1 from TRE-hShh and Pcp2-hShh cerebellum at P6 and P60. Data were represented as mean ± SEM and analyzed by repeated two-way ANOVA and Sidak’s multiple comparisons tests, and n = 5 per group. G Sagittal sections of P6 brains of TRE-LacZ, Camk2a-tTA;TRE-LacZ, Pcp2-tTA;TRE-LacZ, and Camk2a-tTA;Pcp2-tTA;TRE-LacZ mice were analyzed by X-gal staining. The sections were counter-stained with nuclear fast red. H P6 hippocampus from 5 pairs of “TRE-hShh;Gli1-LacZ and Camk2a-hShh;Gli1-LacZ” littermates and P6 cerebellum from 5 pairs of “TRE-hShh;Gli1-LacZ and Pcp2-hShh;Gli1-LacZ” littermates analyzed by Beta-Glo assay. Data were represented as mean ± SEM and analyzed by paired t-tests. See also Additional files 3 (Video S3), 11 (Figure S3).