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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Forebrain Shh overexpression improves cognitive function and locomotor hyperactivity in an aneuploid mouse model of Down syndrome and its euploid littermates

Fig. 1

Ts65Dn shows reduced Gli1 activities in both hippocampus and cerebellum at P6. A X-gal staining of sagittal brain sections of Eu; Gli1-LacZ at P6, P21, and P90. Hippocampus and cerebellum areas were shown. mo, molecular layer; sg, subgranular zone; po, polymorphic layer. Scale bar = 500 um. B Representative images of P6, P21, and P90 of Eu; Gli1-LacZ cerebellum costained for LacZ (black) and Calbindin (green). Nuclear DNA was stained with DAPI (blue). C Detailed analysis of Gli1-positive cells in perinatal and adult cerebellum. Colocalization analysis of white dash labeled areas in (B). At P6, 6 distinct cerebellar cortical layers were found, and from outermost to innermost are Pia (high DAPI, LacZ negative, and Calbindin negative), oEGL (low DAPI, high LacZ, and Calbindin negative), iEGL (high DAPI, low LacZ, and Calbindin negative), PCL (low DAPI, LacZ negative, and Calbindin positive), bPCL (medium DAPI, medium LacZ, and Calbindin negative), and IGL (high DAPI, LacZ negative, and Calbindin negative). P21 or P90 cerebellum has three layers, ML (molecular layer), PCL, and IGL. N ≥ 3 per group (AC). D X-gal staining of P6 sagittal brain sections of Eu;Gli1-LacZ and Ts65Dn;Gli1-LacZ. Hippocampus and cerebellum areas were compared, and n = 3 per group. E Beta-Glo assay of isolated P6 hippocampus and cerebellum from Eu; Gli1-LacZ and Ts65Dn;Gli1-LacZ. Data were represented as mean ± SEM and analyzed by two-way ANOVA and Sidak’s multiple comparisons tests, and n = 4 per group

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