Fig. 1

Workflow for development of histopathological deep learning-based models. (1) Preparation and staining of histopathological tissue sections; (2) Digitization of high resolution whole slide images; (3) Uploading on the Aiforia ® cloud based platform; (4) Annotations on a representative subset (approximately 10%) of the whole dataset and repetitive training of the separate convolutional neural networks (CNNs), that constitute the deep learning-based model (AI-model); (5) Application of the model on the whole dataset; (6) Validation of each CNN; (7) Statistical analysis