Fig. 3

Fraction of GRN expressing cells in the BA10 region and cell-type-specific associated changes in RedPenMC and AMC. A The ratio between number of GRN expressing to non-expressing cells for each cell type in Brodmann area 10 (BA10) sampled from RedPenMC, AMC.26 and NC.38. The P value calculated by comparing different cell types. *indicates P < 0.05. B Different GRN splice variants (S1, S2, S3, S4) expressed in BA10 region of NC (n = 3), AMC (n = 3) and RedPenMC (n = 1) analyzed using Digital Droplet™ PCR (ddPCR). RNA was isolated from the bulk tissue for each donor. GRN splice variants were detected amplifying exon-exon junctions, for S1:E1_E2, S2:E13_3’UTR, S3:E6_E7 and S4:E10_E12. Transcript expression is expressed as number of copies per microliter. The threshold was set automatically above any positive signal detected in multiples negative controls that contained a digital droplet PCR (ddPCR) cocktail and water in place of either of the cDNA. Multiple negative controls were included in each plate. Statistical differences in the expression of four splice variants in NC, AMC and RedPenMC were calculated by one-way ANOVA with Bonferroni's multiple comparison (Post Hoc test). *P < 0.05, ****P < 0.005. C Differentially expressed genes (DEGs) in RedPenMC, AMC.26 and NC.38 (upregulated and downregulated) obtained from all cell types. P values were calculated by comparing the number of DEGs between different cell types per group comparison. *indicates P < 0.05. D The intersection of DEGs in different comparison groups