Fig. 3

Computer-assisted morphometrics to assess pathological tau burden. a, b Quantitative assessment of hyperphosphorylated tau (p-tau) burden was performed on whole slide images of the hippocampus stained for p-tau (AT8) using immunohistochemistry. Positive pixel counts were determined in two regions (hippocampus proper and entorhinal region). Results were normalized to the total area assessed. A third summary score of the total p-tau burden of the medial temporal lobe was calculated by summing positive pixels in both. c High power image shows high intensity in red, medium intensity in yellow and negative staining in blue. d Parallel plot showing the relationship between Braak stage and the computer morphometric quantification of p-tau using the normalized medial temporal lobe (hippocampus and entorhinal region). Scale bar = 150 μm