Table 2 Antibodies used in this study

Primary antibodies

β-actin (C4)

Millipore

MAB1501

Mouse/IgG_{1 k}

43 kD

WB

Pan-Akt (40D4)

Cell Signaling

2920

Mouse/IgG₁

60 kD

WB

Phospho-Akt (Ser473) (D9E)

Cell Signaling

4060

Rabbit/IgG

60 kD

WB

Beta-Amyloid (CT695)

Invitrogen

51–2700

Rabbit/IgG

10–12, 110 kD

WB

Beta-Amyloid_1-16 (6E10)

BioLegend

SIG-39320

Mouse/IgG_{1 k}

4–16, 100 kD

IHC

Soluble APP-alpha (2B3)

Clonetech

11088

Mouse/IgG_2b

100 kD

WB

Soluble APP-beta-swe (6A1)

Clonetech

10321

Mouse/IgG₃

100 kD

WB

ApoE (3B3C32)

BioLegend

674802

Rat/IgG_{2a k}

34 kD

WB

BACE1

Invitrogen

PA1-757

Rabbit/IgG

65 kD

WB

Calnexin (H-70)

Santa Cruz

sc-11397

Rabbit/IgG

90 kD

WB

Phospho-ERK1/2 (Thr202/Tyr204)

Cell Signaling

9101

Rabbit/IgG

42, 44 kD

WB

ERK1/2

Cell Signaling

9102

Rabbit/IgG

42, 44 kD

WB

Farnesyl transferase β subunit (X28)

Santa Cruz

sc-137

Rabbit/IgG

48 kD

WB

GAPDH (V-18), HRP-conjugated

Santa Cruz

sc-20357 HRP

Goat/IgG

36 kD

WB

GAPDH (6C5)

Invitrogen

AM4300

Mouse/IgG₁

36 kD

WB

Geranylgeranyl transferase β subunit (H-3)

Santa Cruz

sc-376655

Mouse/ IgG_{1 k}

42 kD

WB

H-Ras (C20)

Santa Cruz

sc-520

Rabbit/IgG

21 kD

WB

H-Ras

ProteinTech

18295–1-AP

Rabbit/IgG

21 kD

WB

HDJ2 (KA2A5.6)

Invitrogen

MA5-12748

Mouse/IgG1

40 kD

WB

IBA1

Wako

019-19741

Rabbit/IgG

16 kD

IHC

IDE

Sigma-aldrich

AB9210

Rabbit/IgG

110 kD

WB

LRP1 (6F8)

Sigma-aldrich

MABN1796

Mouse/ IgG_{1 k}

85 kD

WB

Neprilysin (F-4)

Santa Cruz

sc-46656

Mouse/ IgG_{1 k}

100 kD

WB

Rab7 (117)

Sigma-aldrich

R8779

Mouse/IgG_2b

24 kD

WB

Rheb (B-12)

Santa Cruz

sc-271509

Mouse/IgG_2b k

21 kD

WB

RhoA (26C4)

Santa Cruz

sc-418

Mouse/IgG_{1 k}

24 kD

WB

p-S6 ribosomal protein (Ser240/244)

Cell signaling

2215

Rabbit/IgG

32 kD

WB

S6 ribosomal protein (5G10)

Cell signaling

2217

Rabbit/IgG

32 kD

WB

Phospho-p70 S6 Kinase (Thr389)(108D2)

Cell signaling

9234

Rabbit/IgG

70, 85 kD

WB

p70 S6 Kinase (49D7)

Cell signaling

2708

Rabbit/IgG

70, 85 kD

WB

α-tubulin (B-5-1-2)

Sigma-aldrich

T5168

Mouse/IgG₁

50 kD

WB

Secondary antibodies/binding proteins

Anti-rabbit IgG, HRP-conjugated

Santa Cruz

sc-2357

Mouse/IgG

WB

Anti-mouse IgG, HRP-conjugated

Santa Cruz

sc-2307

Donkey/IgG

WB

Mouse IgG_k light chain binding protein, HRP-conjugated

Santa Cruz

sc-56102

N/A

WB

Anti-rabbit IgG, HRP-conjugated

Santa Cruz

sc-2305

Donkey/IgG

WB