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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Spinal Muscular Atrophy autophagy profile is tissue-dependent: differential regulation between muscle and motoneurons

Fig. 5

Changes of mTOR protein level and phosphorylation at Ser2448 in SMA spinal cord and isolated MNs. (A) Spinal Cords from P2 and P5 genotyped WT and mutSMA mice were dissected and protein extracts were submitted to western blot analysis using anti-mTOR or anti-phospho-mTOR(Ser2448) antibodies. Membranes were reprobed with anti-α-tubulin antibody. Graph values represent the expression of mTOR or p-mTOR versus α-tubulin and correspond to the quantification of at least five independents experiments ± SEM. Asterisks indicate significant differences using Student t test (*p < 0.05; **p < 0.01; no significant differences (ns) p > 0.05). Protein extracts of 6-day cultured WT and mutSMA mice MNs (B) and 7-day differentiated Control and SMA human MNs (C) were submitted to western blot analysis using anti anti-mTOR or anti-phospho-mTOR(Ser2448) antibodies. Membranes were reprobed with anti-α-tubulin antibody. Graph values represent the expression of mTOR or phospho-mTOR(Ser2448) (p-mTOR) versus α-tubulin and correspond to the quantification of at least four independent experiments ± SEM. Asterisks indicate significant differences using Student t test (*p < 0.05; no significant differences (ns) p > 0.05)

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