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Table 2 Antibodies

From: Pathological tau drives ectopic nuclear speckle scaffold protein SRRM2 accumulation in neuron cytoplasm in Alzheimer’s disease

Antigen Clone/product name Dilution Host species Source Catalog #
pSRRM2 SC-35 1:200 Mouse SigmaAldrich S4045
pTau
Thr231
AT180 1:200 Mouse ThermoScientific (Rockford, IL, USA) MN1040
NeuN A60 1:400 mouse Millipore MAB377
Tau (Total) SP70 pAb 1:1,000 Rabbit Rockland Immunochemicals Inc. (Limerick, PA, USA) 200-C01-B33
pTau Ser202 CP13 mAb 1:500 Mouse Peter Davies (Litwin-Zucker Research Center for the Study of Alzheimer's Disease, The Feinstein Institute of Medical Research, Northwell Health, Manhasset, NY, USA) N/A
pTau Ser396/Ser404 PHF-1 mAb 1:2,000 Mouse
pTau
Ser422
EPR2866 mAb 1:500 Rabbit Abcam (Cambridge, UK) ab79415
pTau Thr181 AT270 mAb 1:15,000 Mouse ThermoFisher Scientific (Waltham, MA, USA) MN1050
Tau (Total) K9JA (DAKO) pAb 1:250 Rabbit Agilent Technologies, Inc. (Santa Clara, CA, USA) A002401-2
2° Ab Mouse Horseradish Peroxidase α-Ms IgG (H + L) 1:5,000 Goat Jackson Immunoresearch (West Grove, PA, USA) 115-035-146
2° Ab Rabbit Horseradish Peroxidase α-Rb IgG (H + L) 1:5,000 Goat Jackson Immunoresearch (West Grove, PA, USA) 111-035-144
2° Ab Mouse Alexa Fluor® 568 Goat α-Ms IgG (H + L) 1:1,000 Goat Invitrogen (Carlsbad, CA, USA) A-11004
2° Ab Rabbit Alexa Fluor® 647 Goat α-Rb IgG (H + L) 1:1,000 Goat Invitrogen (Carlsbad, CA, USA) A-21245