Table 2 Antibodies
Antigen | Clone/product name | Dilution | Host species | Source | Catalog # |
|---|---|---|---|---|---|
pSRRM2 | SC-35 | 1:200 | Mouse | SigmaAldrich | S4045 |
pTau Thr231 | AT180 | 1:200 | Mouse | ThermoScientific (Rockford, IL, USA) | MN1040 |
NeuN | A60 | 1:400 | mouse | Millipore | MAB377 |
Tau (Total) | SP70 pAb | 1:1,000 | Rabbit | Rockland Immunochemicals Inc. (Limerick, PA, USA) | 200-C01-B33 |
pTau Ser202 | CP13 mAb | 1:500 | Mouse | Peter Davies (Litwin-Zucker Research Center for the Study of Alzheimer's Disease, The Feinstein Institute of Medical Research, Northwell Health, Manhasset, NY, USA) | N/A |
pTau Ser396/Ser404 | PHF-1 mAb | 1:2,000 | Mouse | ||
pTau Ser422 | EPR2866 mAb | 1:500 | Rabbit | Abcam (Cambridge, UK) | ab79415 |
pTau Thr181 | AT270 mAb | 1:15,000 | Mouse | ThermoFisher Scientific (Waltham, MA, USA) | MN1050 |
Tau (Total) | K9JA (DAKO) pAb | 1:250 | Rabbit | Agilent Technologies, Inc. (Santa Clara, CA, USA) | A002401-2 |
2° Ab Mouse | Horseradish Peroxidase α-Ms IgG (H + L) | 1:5,000 | Goat | Jackson Immunoresearch (West Grove, PA, USA) | 115-035-146 |
2° Ab Rabbit | Horseradish Peroxidase α-Rb IgG (H + L) | 1:5,000 | Goat | Jackson Immunoresearch (West Grove, PA, USA) | 111-035-144 |
2° Ab Mouse | Alexa Fluor® 568 Goat α-Ms IgG (H + L) | 1:1,000 | Goat | Invitrogen (Carlsbad, CA, USA) | A-11004 |
2° Ab Rabbit | Alexa Fluor® 647 Goat α-Rb IgG (H + L) | 1:1,000 | Goat | Invitrogen (Carlsbad, CA, USA) | A-21245 |