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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: RBM5-AS1 promotes radioresistance in medulloblastoma through stabilization of SIRT6 protein

Fig. 2

RBM5-AS1 contributes to medulloblastoma stemness. A Western blot analysis of the stem cell marker CD133 in radioresistant and parental DAOY cells transfected with control shRNA (shCtrl) or shRBM5-AS1. B Quantitative real-time PCR analysis of CD44 and SOX2 in radioresistant and parental DAOY cells transfected with indicated constructs. C Radioresistant and parental DAOY cells transfected with shCtrl or shRBM5-AS1 were cultured in the suspension condition to allow the formation of tumorspheres. Top panels: representative photographs of tumorspheres. Scale bar = 40 μm. *P < 0.05

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