Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer’s disease

Emre, Ceren; Do, Khanh V.; Jun, Bokkyoo; Hjorth, Erik; Alcalde, Silvia Gómez; Kautzmann, Marie-Audrey I.; Gordon, William C.; Nilsson, Per; Bazan, Nicolas G.; Schultzberg, Marianne

doi:10.1186/s40478-021-01216-4

Table 2 Cytokines and chemokines detected in brain homogenates. Statistical analysis between WT and APP KI per age group

Cortex	Median (pg/ml)				Median (pg/ml)				Median (pg/ml)				Median (pg/ml)				LLOD	LLOQ
	2mo				4mo				8mo				18mo				pg/ml	pg/ml
	P value	WT	APP KI		P value	WT	APP KI		P value	WT	APP KI		P value	WT	WT	APP KI	pg/ml	pg/ml
IL-5	0.009	0.368	0.553	↑	0.18	1.135	0.64	↓	0.999	0.301	0.301		0.002	1.075	1.422	↑	0.06	0.302
IL-4	0.03	0.338	0.461	↑	0.589	0.357	0.334	↓	0.143	0.264	0.304	↑	0.002	0.336	0.463	↑	0.11	0.818
IL-10	N/A	NA	NA		0 .818	2.034	2.098	↑	NA	NA	NA		0.065	1.849	2.23	↑	0.94	7.26
IL-27p28/1L-30	0.818	16.45	15.83	↓	0.041	6.471	4.711	↓	0.589	11.14	9.958	↓	0.041	19.26	25.98	↑	1.39	5.91
TNF-α	0.029	0.286	0.383	↑	0.394	0.833	0.685	↓	0.009	0.183	0.46	↑	0.004	0.847	2.041	↑	0.13	980
KC-GRO	0.002	3.092	5.03	↑	0.002	30.47	11.66	↓	0.026	4.811	7.914	↑	0.002	13.57	26.45	↑	0.24	3.29
IL-1β	0.065	0.434	0.533	↑	0.24	0.587	0.695	↑	0.065	0.712	1.865	↑	0.002	0.816	5.292	↑	0.11	0.72
IL-6	0.026	11.85	17.52	↑	0.041	33.73	18.88	↓	0.699	7.453	7.908	↑	0.002	29.61	39.1	↑	0.61	7.61
IL-2	0.052	0.502	0.910	↑	0.132	1.399	0.825	↓	0.143	0.491	0.588	↑	0.002	1.107	1.559	↑	0.22	1.03
IL-12p70	0.004	54.45	78.36	↑	0.31	125.1	72.88	↓	0.589	24.36	31.69	↑	0.026	106.5	129.1	↑	9.95	179
IFN-y	0.009	0.226	0.31	↑	0.121	0.086	0.045	↓	0.258	0.212	0.191	↓	0.002	0.07	0.12	↑	0.04	0.39
MIP-2	0.18	1.054	1.313	↑	0.004	2.973	1.4	↓	0.002	1.243	2.401	↑	0.002	2.395	5.52	↑	0.053	0.58
IP-10	0.093	6.532	5.619	↓	0.485	11.98	13.33	↑	0.002	6.189	33.32	↑	0.002	9.174	109.8	↑	0.328	2.15
MIP-1α	0.18	8.51	9.634	↑	0.002	6.763	61.42	↑	0.002	5.454	167.1	↑	0.002	11.03	353.3	↑	0.081	0.38
IL-15	0.818	176.4	174.2	↓	0.002	72.01	49.71	↓	0.818	176.5	165.6	↓	0.041	233.5	296.1	↑	16	43.2
IL-17A/F	0.513	6.004	6.231	↑	0.004	6.024	3.43	↓	0.485	6.001	4.217	↓	0.041	9.669	11.7	↑	0.231	1.39
MCP-1	0.132	31.495	33.37	↑	0.286	13.32	14.95	↑	0.132	27.6	21.52	↓	0.004	37.3	51.76	↑	0.672	4.42
IL-9	0.31	10.743	12.11	↑	0.009	16.28	11.99	↓	0.222	11.52	7.516	↓	0.065	16.55	19.64	↑	3.84	21.9
IL-33	0.31	251.58	285.6	↑	0.18	156.1	190.2	↑	0.18	359.4	499.1	↑	0.002	521.8	2016.4	↑	0.364	1.85

Hippocampus	Median (pg/ml)				Median (pg/ml)				Median (pg/ml)				Median (pg/ml)
	2mo				4mo				8mo				18mo
	P value	WT	APP KI		P value	WT	APP KI		P value	WT	APP KI		P value	WT	APP KI
IL-5	0.18	0.389	0.468	↑	0.093	0.411	0.496	↑	0.24	0.214	0.231	↑	0.394	0.518	0.54	↑	0.06	302
IL-4	0.998	0.378	0.39	↑	0.394	0.335	0.372	↑	0.307	0.188	0.202	↑	0.784	0.366	0.36	↓	0. 11	0.818
IL-10	NA	NA	NA		NA	NA	NA		NA	NA	NA		NA	NA	NA		0.9.4	7.26
IL-27p28/1L-30	0.132	12.79	15.96	↑	0.937	12.52	13.47	↑	0.178	5.8	7.724	↑	0.041	20.38	24.69	↑	1.39	5.91
TNF-α	0.065	0.201	0.282	↑	0.084	0.258	0.319	↑	0.191	0.13	0.198	↑	0.002	0.413	1.126	↑	0.13	0.98
KC-GRO	0.18	5.067	6.519	↑	0.002	14.14	5.7	↓	0.394	3.548	5.757	↑	0.009	8.711	14.45	↑	0. 24	3.29
IL-1β	0.31	0.32	0.387	↑	0.026	0.369	0.509	↑	0.24	0.277	0.44	↑	0.002	0.851	3.053	↑	0.11	0.72
IL-6	0.165	11.87	15.05	↑	0.18	13.9	16.86	↑	0.792	5.006	5.566	↑	0.18	17.02	19.71	↑	0.61	7.61
IL-2	0.455	0.666	0.757	↑	0.699	0.614	0.659	↑	0.571	0.282	0.315	↑	0.937	0.726	0.754	↑	0.22	1.03
IL-12p70	0.394	46.42	49.82	↑	0.699	45.81	46.28	↑	0.571	9.232	11.074	↑	0.784	56.46	53.27	↓	9.95	179
IFN-y	0.288	0.241	0.253	↑	0.104	0.211	0.25	↑	0.485	0.147	0.158	↑	0.197	0.262	0.279	↑	0.04	390
MIP-2	0.31	0.861	0.942	↑	0.041	2.505	1.216	↓	0.31	0.517	0.991	↑	0.002	2.466	4.595	↑	0.053	0.58
IP-10	0.31	9.215	8.406	↓	0.699	9.973	10.34	↑	0.004	3.636	15.35	↑	0.002	12.49	88.79	↑	328	2.15
MIP-1α	0.589	3.414	3.968	↑	0.002	3.416	19.48	↑	0.002	1.928	54.22	↑	0.002	9.231	304.9	↑	0.081	0.38
IL-15	0.24	154.9	172.6	↑	0.818	190.5	166.9	↓	0.931	69.34	80.13	↑	0.026	254.8	322.1	↑	16	43.2
IL-17A/F	0.041	4.377	5.543	↑	0.31	8.042	6.996	↓	0.444	1.652	2.184	↑	0.002	9.737	13.16	↑	0.231	1.39
MCP-1	0.818	23.81	25.46	↑	0.589	27.97	26.96	↓	0.485	16.35	22.25	↑	0.015	33.78	45.04	↑	672	4.42
IL-9	0.065	7.477	11.28	↑	0.937	13.36	14.05	↑	0.485	8.438	10.31	↑	0.169	18.89	21.93	↑	3. 84	21.9
IL-33	0.24	231.3	294.7	↑	0.041	305.9	457.7	↑	0.132	294.2	457	↑	0.002	1030	2473	↑	0.364	1 _85

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