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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Inducing sterile pyramidal neuronal death in mice to model distinct aspects of gray matter encephalitis

Fig. 5

Indicators of neuronal activity and circuitry in the hippocampus upon complex wheel running. a Schematic experimental outline. Approximately 2 months after 3 × tamoxifen induction mice were challenged for 4 h exposure to voluntary complex wheel running to induce neuronal activation. b Representative images of cFos (white) and PV (red) double staining in control and DTA mice. c Quantification of total number of cFos+ cells in hippocampal regions upon CRW. DTA mice showed significantly reduced numbers of cFos+ cells in HC and its CA regions, whereas the dentate gyrus (DG) remained unaffected. d PV+ interneuron numbers 2 months after ablation of pyramidal neurons. DTA mice displayed increased PV+ interneuron numbers in HC and its CA regions, but not in DG. e Evaluation of PV+ interneuron activation upon CRW. PV+ interneurons were less frequently activated (evaluated by cFos expression) in CA3 region of DTA mice, with similar tendency in CA1. DG was again similar between both groups. Data from 8 mice/group displayed as mean ± SD; 2-tailed unpaired Welch’s corrected t-tests or Mann–Whitney U-tests

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