Fig. 5

The endosome pathway appears affected in muscle from patients with hypokalemic periodic paralysis. a. The early endosome markers EEA1 and rab5 appear to accumulate in vacuoles, while the late endosome marker rab7 and endosome recycling marker rab11 does not, suggesting intermittent endosome processing arrests. b. The autophagosomal pathway appears affected in muscle from patients with hypokalemic periodic paralysis. The autophagophore markers p62/SQSTM1 and beclin-1 clearly accumulate in some vacuoles while LC3 often appears dissociated from p62 and Atg5, a late autophagosomal marker does not accumulate in the vacuoles at all. Like for endosome processing, the autophagosomal marker stains suggest an intermittent processing arrest between early and late stage. Bar is 50 µm