Fig. 5
From: α-Synuclein pathology in Parkinson disease activates homeostatic NRF2 anti-oxidant response
Quantitative RT-PCR analyses of anti-oxidant gene response in the brain regions of PBS or PFF aSyn injected M83+/+ mice. A Keap1 (Kelch Like ECH Associated Protein 1, NRF2 inhibitor protein), and B–D NRF2 anti-oxidant response mediators, Hmox1 (Heme Oxygenase 1, in B, Gclc (glutamate–cysteine ligase catalytic subunit, in C and Gsr1 (glutathione–disulfide reductase, in D. Relative mRNA quantitation at terminal stage in the following brain regions: frontal cortex (Ctx), thalamus (Thal), corpus striatum (Str), hippocampus (Hip), midbrain (Mb) pons, medulla (Med) and cerebellum (Crb). Mouse Gapdh was used as a reference gene (ΔΔCT method: n = 6/group, samples were run in duplicates; Error bars represent standard error of the mean, s.e.m.; Pair-wise comparisons were assessed by Mann–Whitney test-only significant differences (* = p ≤ 0.05) are highlighted (ns = not significant); Multiple columns comparisons in One way ANOVA post-hoc Bonferroni test were not significant). Also see Additional file 1: Fig. S7