Fig. 3

GBM CSCs and non-CSCs have differential levels of lipid accumulation. A Dissociated cells from GBM PDX models were stained for the stem cell marker CD133 and magnetically sorted. B Oil Red O staining was higher in the cultured non-CSC population than in the CSC population. C The CD133-negative non-CSC population showed increased fluorescence compared to CD133-positive CSCs for both Nile red and BODIPY lipid-specific fluorescent dyes. D Magnetically sorted CSC and non-CSC populations were subsequently FACS sorted into lipid-high and lipid-low populations based on BODIPY fluorescence (top and bottom 20%). Sphere-forming capability was determined by limiting-dilution assay. Non-CSCs showed uniformly low sphere-forming capability. BODIPY-low CSCs had significantly higher self-renewal capacity than their BODIPY-high counterparts (p = 0.002). Calculated stem cell frequencies and 95% confidence intervals are shown