Fig. 7
scRNA-Seq analysis of tau-seeded F+/T+ brains upon PLX3397 treatment. a Schematic overview of scRNA-Seq analysis in tau-seeded F+/T+ mice that were or were not treated with PLX3397 for 1.5 months following tau-seeding. Brains were collected from n = 2 mice for each condition. b UMAP-projection containing 4102 CD45+ cells originating from tau-seeded F+/T+ mice which had received control chow following tau-seeding, and 2130 CD45+ cells originating from tau-seeded F+/T+ mice which had received PLX chow following tau-seeding to deplete microglia. Clusters 1 to 5 correspond to different activation states of microglia population. c UMAP plots showing the expression of homeostatic microglia marker genes (top) and DAM marker genes (bottom). d Pie charts showing the percentages of each of the CD45+ subsets within the total CD45+ fraction in control (left) and PLX3397 treated (right) tau-seeded F+/T+ brains. e UMAP plots of the merged dataset of the control treated (left) and PLX3397 treated (right) tau-seeded F+/T+ whole brains as shown in b, where each UMAP plot highlights the cells from each condition separately. Clusters are colored according to the cluster colors shown in b. Only cells belonging to one of the 2 treatment conditions are colored per panel, cells belonging to the other treatment condition are depicted in grey. f Bar graphs showing the percentages of microglia subsets within total microglia per treatment group (red: control, blue: PLX)