Fig. 1

Overview of differentiation protocol and identification of the iPSCs-derived human-like dopaminergic neurons at early stage of differentiation. a Schematic depicts the timeline of differentiation protocol, growth factors/small molecules and culture medium used for differentiation. b Immunostaining for markers of dopaminergic neurons and α-synuclein after 36 days of differentiation. Cells were subject to immunostaining for two markers of dopaminergic neurons the dopamine transporter (DAT) and tyrosine hydroxylase (TH). Scale bar: 100 μm- All experiments and analyses are performed via a blinded experimental design