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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration

Fig. 7

Reduced EU-labelling in Purkinje cells of ATXN1[82Q] mice. a, b Confocal images (maximum projection of 10 optical sections, 4.5-μm stacks; inserts are from single optical sections) illustrating EU labelling in Purkinje cells and Bergmann glia from 9-week-old control and ATXN1[82Q] mice. Animals were perfused 1 h following EU injection. Note that the orientation of the images is parallel to the Purkinje cell layer. Also note large vacuoles in a ATXN1[82Q] Purkinje cell (arrows in insert ii). cf Graphs showing relative intensities of nuclear EU labelling (c), nuclear volume (d), relative EU intensity × nuclear volume (e); and intensity of nucleolar EU labelling relative to EU intensity of the rest of the nucleoplasm (f) in control and ATXN1[82Q] Purkinje cells. Bar graphs (upper row) show means ± SE per animal (n = 3). P values are from 2-tailed Student's t-tests. *P < 0.05. Violin plots show values of individual Purkinje cells. Inside the violin plots, the black lines indicate the median of the data and the interquartile range (25–75% data range). The contour line represents the 95% confidence interval. ***P < 0.001; ****P < 0.0001; Welch t-tests. Scale bars: 20 μm

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