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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration

Fig. 3

Subcellular distribution of EU in cerebellar neurons. ae Representative high magnification confocal images (optical section 1 μm) of EU labelling in nuclei of Purkinje cells (a, d, e), Bergmann glia (a′, d′, e′), a molecular layer interneuron (b) and granule cells (c) at 1, 6 and 24 h after EU injection. Relative higher levels of EU labelling occur in nucleoli of Purkinje cells and other cerebellar cell types 1 h post injection (arrows in ad). No increased nucleolar EU-labelling was found 24 h post-injection (arrow in e). Note that image in a′ is the same as in a but collected with different detector settings resulting in overexposure of Purkinje cells, but better visualisation of EU signal in Bergmann glia. f, g Co-staining of EU, DAPI and Sf3a showing lower EU signal in DAPI-/Sf3a + domains of the nucleoplasm (arrow heads). Scale bars: 10 μm (a′) and 5 μm (c, g)

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