Fig. 3From: In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegenerationSubcellular distribution of EU in cerebellar neurons. a–e Representative high magnification confocal images (optical section 1 μm) of EU labelling in nuclei of Purkinje cells (a, d, e), Bergmann glia (a′, d′, e′), a molecular layer interneuron (b) and granule cells (c) at 1, 6 and 24 h after EU injection. Relative higher levels of EU labelling occur in nucleoli of Purkinje cells and other cerebellar cell types 1 h post injection (arrows in a–d). No increased nucleolar EU-labelling was found 24 h post-injection (arrow in e). Note that image in a′ is the same as in a but collected with different detector settings resulting in overexposure of Purkinje cells, but better visualisation of EU signal in Bergmann glia. f, g Co-staining of EU, DAPI and Sf3a showing lower EU signal in DAPI-/Sf3a + domains of the nucleoplasm (arrow heads). Scale bars: 10 μm (a′) and 5 μm (c, g)Back to article page