Fig. 6

rTMS prevents decline of neuronal activity in the 5xFAD brains. a Representative images of c-FOS staining in the mPFC. Scale bar, 100 μm. b Quantitative analyses of the density of c-FOS positive cells in the mPFC across groups (n = 8–10 mice per group, 98 ± 16 cells per section were sampled for the analysis). c Representative images of c-FOS staining in different subregions of dorsal hippocampus. Scale bar, 100 μm. d Quantitative analyses of the density of c-FOS positive cells in different subregions of hippocampus across groups (n = 8–11 mice per group, 95 ± 6, 89 ± 22 and 95 ± 21 cells were sampled from DG, CA3 and CA1 brain regions for analysis). e Representative images of c-FOS staining in the S1 cortex. Scale bar, 150 μm. f Quantitative analyses of the density of c-FOS positive cells in the S1 cortex across groups (n = 8–11 mice per group, 414 ± 28 cells per section were sampled for the analysis). All data are presented as mean ± SEM of the fold change of the WT-Ctrl group and analyzed by one-way ANOVA followed by Tukey's multiple comparisons test. WT-Ctrl: wildtype littermates received sham treatment, AD-Ctrl: 5xFAD mice received sham treatment, AD-rTMS: 5xFAD mice received rTMS treatment. mPFC: medial prefrontal cortex, DG: dentate gyrus, CA3: cornu ammonis 3, CA1: cornu ammonis 1, S1: primary sensory cortex