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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Zika virus induces neuronal and vascular degeneration in developing mouse retina

Fig. 5

ZIKV induces subtle retinal pathology at P8. ZIKV was injected into neonatal mice at P0 and retinas were collected at P8. a Representative images of PI-stained retinal sections at P8. Graph represents the thickness of individual retinal layers and total retina from GCL to ONL. n = 5 per group. b Representative images of retinal flatmounts labeled with isolectin B4 for vasculature. Arrows indicate neovascular tufts. Graphs represent vascular area (n = 11–14 per group) and the density of vessel branching points (n = 7–8 per group). cg Representative images of retinal flatmounts labeled with anti-RBPMs and Tuj1 for RGCs and their axons, anti-GFAP for astrocytes, anti-Iba1 for microglia, and anti-CD45 for leukocytes. hj Graphs represent the quantification of numbers of RGCs (n = 6–7 per group), microglia and leukocytes (n = 5 per group). Scale bar = 50 μm except the upper panel in b where scale bar = 500 μm. *p < 0.05, ***p < 0.001 compared to control mice

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