Fig. 6

Sarm1 knockout normalizes time spent sleeping in the chronic stage of TBI: mice were single housed in cages equipped with a PiezoSleep mouse behavioral tracking system for 72 h during the eighth week after TBI. a–b Mice were acclimated for 18 h. Statistical analysis focused on data collected during 48 h across two complete cycles of lights on (white bars) and lights off (dark bars and gray background). Recordings continued for a subsequent 6 h to show the final wake/sleep transition. In Sarm1 WT mice (a), the time spent sleeping per hour is significantly different between sham and TBI, with the injured mice appearing to sleep less during the normal sleeping period when lights are on. In Sarm1 KO mice (b) the sleep pattern was not different between sham and TBI conditions during the 48 h data collection period. c TBI significantly reduced the time spent sleeping for Sarm1 WT mice during the normal sleeping period when lights are on. Sarm1 KO mice did not exhibit sleep loss after TBI. d During the dark period, sleep time was not different based on injury or genotype. Sarm1 WT: n = 8 sham, n = 10 TBI. Sarm1 KO: n = 9 sham, n = 10 TBI. ns = not significant. Further statistical details are provided in Additional File 1: Table S5