Fig. 8

Change in cellular localization of AQP4 in prion-infected mice. (a) Reduction of perivascular AQP4 and increase of non-perivascular AQP4 in the cortex of SSLOW-infected mice (top) in comparison to age-matched control mice (bottom). (b) Normalized count of AQP4 mRNA in cortex plotted for different groups of mice. NanoString data were plotted as group averages with standard deviations (n = 6 for normal females, and n = 3 for other groups). (c) Ratio of perivascular versus non-perivascular AQP4 immunofluorescence signal in cortex of SSLOW and age-matched control mice. (d) Higher magnification images of cortical vessels in age-matched control (upper row) and SSLOW-infected mice (lower row) co-immunostained for GFAP (green) and AQP4 (red). (e) Representative profiles across microvessels in the cortices of age-matched control (upper plot) and SSLOW-infected mice (lower plot). (f) AQP4 immunoreactivity outlines hypertrophic astrocytes of SSLOW-infected mice. Scale bar 60 µm in a, 20 µm in d and f