Fig. 7
From: The degree of astrocyte activation is predictive of the incubation time to prion disease
Immunoreactivity of vimentin in SSLOW-infected animals. Co-immunostaining of SSLOW-infected mice and age-matched control for vimentin (VIM, red), GFAP (green) and nuclei (DAPI, blue). (a) Similar to control mice (right), SSLOW-infected mice (left) maintain strong Vimentin immunoreactivity of ependymal cells. (b, c) Astrocytes in normal animals lacked vimentin immunoreactivity (b, top). In SSLOW-infected animals, hypertrophic GFAP + astrocytes consisted of heterogeneous VIM+ and VIM− sub-populations (b, bottom and c). Scale bar: 60 μm in a, 20 µm in b and c. Asterisks: non-specific binding of anti-Vim antibodies to nuclei