Fig. 1
Spinal homogenates from paralyzed G37R SOD1 mice seed G85R-SOD1:YFP mice inefficiently with variable incubation periods. a Scatter plot of the ages at which G85R-SOD1:YFP mice developed paralysis or were euthanized after injection of spinal homogenates. Scatter plots in this figure and figures that follow were generated in GraphPad Prism v9. TgG37R denotes spinal homogenate from paralyzed G37R-Line 29 mice. P1-G37RG85R−SOD1:YFP denotes inoculum from a paralyzed first-passage G85R-SOD1:YFP animal. P2-G37RG85R−SOD1:YFP denotes inoculum prepared from a paralyzed second-passage G85R-SOD1:YFP animal. The dashed lines with arrows mark animals from the initial passage that were used to prepare inoculum for second passage. b–h Representative images of inclusion pathology in cryostat sections that were induced in the spinal cord of G85R-SOD1:YFP mice that had been injected intraspinally (ISP) with spinal homogenates from G37R mice with subsequent passages (images representative of 2–3 sections per mouse). The arrows identify pathological specimens that are related due to passage. b–d Pathology specimens from first passage animals. e–g Representative pathology specimens from 2nd passage animals. h A representative image of pathology specimens from 3rd passage animals. Raw data provided in Additional file 1: Data File 1. Scale bars = 50 µm