Fig. 7
From: Aβ43 aggregates exhibit enhanced prion-like seeding activity in mice
Induction of Aβ CAA in Aβ43-inoculated AppNL−F mice. a Schematic of the location of Aβ CAA in the brains of inoculated AppNL−F mice. b Quantification of Aβ42 CAA-positive leptomeningeal arteries (12F4 immunostaining) in AppNL−F mice at 6 months post-inoculation with either Aβ38 (n = 6), Aβ40 (n = 8), Aβ42 (n = 10), Aβ43 (n = 8), TgCRND8 (n = 9), or AppNL−F Aβ (n = 9). Mice inoculated with either dH2O (n = 5) or material derived from a non-Tg mouse brain (n = 6) were used as negative controls. The extent of Aβ CAA was significantly higher in mice injected with recombinant Aβ43 aggregates or brain-derived Aβ aggregates compared to mice injected with dH2O (P = 0.00040 for Aβ43, P = 0.043 for TgCRND8, and P = 0.0019 for AppNL−F as determined by a Kruskal–Wallis test followed by Dunn’s multiple comparisons test; all other groups non-significant compared to dH2O-injected mice). Open circles indicate female animals and filled circles indicate male animals. c Representative images of leptomeningeal (top row) and cortical (bottom row) Aβ42 CAA (12F4 immunostaining) in AppNL−F mice at 6 months post-inoculation with the indicated Aβ preparations. Scale bar = 20 µm (applies to all images)