Skip to main content
Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Combined epigenetic/genetic study identified an ALS age of onset modifier

Fig. 2

Genome-wide DNA methylation (DNAm) analysis and genetic association study suggested candidate CpG-SNPs associated with ALS age of onset.  a Manhattan plot presenting the association between DNAm levels of common CpG-SNPs and ALS age of onset. Three CpG-SNPs in blue remained significant after adjusting for sex, site of onset, DNAm-age acceleration and the top 5 principal components. The blue line represents the trend of statistical significance (false discovery rate < 0.05). b The locus on chr1:151,150,000–151,246,000 tagged by rs4970944 associated with ALS age of onset. Arrows indicate the transcriptional direction of VPS72 (5′ to 3′). “Me” in red represents the CpG-site, methylation of which is affected by rs4970944. The linkage disequilibrium (LD) block tagged by rs4970944 (R2 > 0.8) is highlighted in green. c Genotypes of rs4970944 are significantly associated with DNAm status: P = 2 × 10−16, B = -0.25 (SE: 0.003); and age of onset: P = 0.003 adjusted for sex, family history, site of onset and DNAm-age acceleration, B = 3.0 (SE: 1.0). The dashed line represents the linear regression trend

Back to article page