Fig. 4

Proteomic analysis of Vps13a^−/− mouse basal ganglia revealed accumulation of neurotoxic proteins related to impaired autophagy. a Heatmaps of statistically relevant identified proteins. Each line corresponds to a protein and each column is relative to a different sample. The different coloration is dependent on quantity of protein present in sample based on the statistical performed analysis. Specifically, red color refers to up-regulated proteins while green is associated to down-regulated proteins. The logarithm Fold Change scale is also reported. Panel A and panel B report proteins down-regulated and up-regulated in Vps13a^−/− mice compared to WT, respectively. b Upper panel. Western blot (Wb) analysis of γ-Synuclein, synaptotagmin and Rab 3 in isolated basal ganglia from 12 and 18 months (Mo) old wild-type and Vps13a^−/− mice. GAPDH was the protein loading control. Middle panel. Densitometric analyses of the immunoblot bands similar to those shown are presented in bar graph. Data are means ± SEM (n = 6; * P < 0.02 ChAc vs. WT by t-test). Lower panel. Representative confocal images of the γ-synuclein protein (green) in the striatum of WT and Vps13a^−/− mice at 18 months of age. Boxplots summarize the results presented as mean ± standard deviation (n = 3 animals for group; * P = 0.029). c Western blot (Wb) analysis of phopho-tau At8, At180, and total tau in isolated basal ganglia from 12 and 18 months (Mo) old wild-type and Vps13a^−/− mice. GAPDH was the protein loading control. Densitometric analyses of the immunoblot bands similar to those shown are presented at right. Data are means ± SEM (n = 6; *p < 0.02 vs. WT by t-test)