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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Therapeutic targeting of Lyn kinase to treat chorea-acanthocytosis

Fig. 2

Vps13a−/− mice show neuronal loss associated with signs of neuroinflammation. a N-Acetyl Aspartate (NAA) concentration determined by 1H MRS (7 T) in the striatum of Vps13a−/− or WT control mice at different age (7, 12 and 18 months). NAA concentration was normalized using creatine as internal reference. Data are mean ± SEM (*p < 0.05 by t-test vs. WT). b Representative images of NeuN staining in cortex of WT control and Vps13a−/− mice at 12 months of age. Neurons in green, nuclei in blue. Scale bar: 50 mm (Objective 20x). Quantification of NeuN-positive cells area in cortex. Results are expressed as mean ± SEM. c Representative images of Iba-1 positive microglia cells in cortex of WT control and Vps13a−/− mice at 12 months of age (Microglia in red, nuclei in blue). Scale bar:50 mm. Quantitative analysis of microglia show significant differences in microglial density and activation in the cortex of Vps13a−/− compared to WT control mice. Results are expressed as mean ± SEM (****P < 0.0001; Unpaired t-test) d Western blot (Wb) analysis of phospho-NF-kB and total NF-kB in isolated basal ganglia of wild-type (WT) and Vps13a−/− mice at 12 and 18 months (Mo) of age. GADPH is the loading control. Densitometric analysis is shown in Additional file 1: Fig. S6. e mRNA expression of interleukine-1β (Il-1b) by qRT-PCR on cortex and basal ganglia from 12 and 18 months (Mo) old WT and Vps13a−/− mice. Experiments were performed in triplicate. Data are mean ± SD. *P < 0.05 compared with WT mice using ANOVA; internal comparisons were calculated by unpaired student t-test

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