Skip to main content
Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson’s disease and dementia with Lewy bodies

Fig. 1

αSyn RT-QuIC analysis of PD and DLB brain samples. a αSyn RT-QuIC spectra of BH from neuropathologically confirmed cases of PD (n = 3, left panels), DLB (n = 3, middle panels), and NS controls (n = 3, right panels). Case numbers were indicated in parentheses. Two µl of BH diluted to 10–3 through 10–8 (w/v) was used for seeding RT-QuIC reactions in quadruplicate. Average RT-QuIC reactivity was shown for individual BH dilutions of each of 3 cases tested in quadruplicate. Data were expressed as percentages of the maximum ThT fluorescence (left Y-axis), with corresponding relative fluorescence units (rfu, right Y-axis). b Lag phase of RT-QuIC reactions from individual dilutions of PD, DLB, and control samples. RT-QuIC spectra for individual dilutions of each case in a were used to obtain the time (h) required for the average fluorescence to excess the threshold of RT-QuIC reactions (11% or 30,000 rfu). For negative reactions that did not reach threshold during the assay, lag phase was assigned as 60 h. Shown were individual time points with error bars of means ± S.E. plotted against log-dilution series. c Protein aggregation rate (PAR) of RT-QuIC reactions from individual dilutions of PD, DLB, and control samples. Lag phase data (h) in b were converted to PAR (1/h). Shown were average rate values and error bars of S.E. Semi-log linear regression lines were applied to the PD (r2 = 0.91) and DLB (r2 = 0.88) groups. For negative reactions (assigned with a lag phase of 60 h), the rate was set at 0 (dotted line). **p < 0.01, ***p < 0.005, ****p < 0.001

Back to article page