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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Amyloid precursor protein elevates fusion of promyelocytic leukemia nuclear bodies in human hippocampal areas with high plaque load

Fig. 1

Dynamic nuclear aggregates are present in various cells, lack a membrane coating and are transcriptionally active. (A) Upon co-expression of FE65-EGFP (green) and TIP60-HA (w/o fluorophore, stained using anti-HA tag antibody (red)), nuclear aggregates in various sizes are generated in multiple cell lines including neurons (for neurons no co-staining was done as a mCherry vector was additionally co-transfected to identify neuronal cell structure). Transfected vectors with respective fluorophore (EGFP, mCherry) are indicated. (B) Every cell type used in A demonstrated cells with many tiny (arrow) or few large spheres (arrowhead) (or transition states) supporting the hypothesis of sphere fusion over time (blue, APP-CT50; red, FE65-mCherry, TIP60-HA un-stained; a different overview image is also given in Additional file 1: Fig. S1). (C) Transmission electron microscopy of FE65/TIP60-HA transfected cells revealed an electron-dense ring structure (additional image in Figure S2). However, there was no evidence for a membrane sheath. Additionally, these results were also confirmed by a CellMask staining (Additional file 1: Fig. S3). (D) High-resolution STED imaging revealed that the inner core of the aggregates is positive for anti K9 acetylation histone 3 antibody staining (red, RFP) supporting the hypothesis of active gene expression within the aggregates

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