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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Robust α-synuclein pathology in select brainstem neuronal populations is a potential instigator of multiple system atrophy

Fig. 5

Biochemical fractionation followed by Western blot analysis of cerebellum and pons white matter from control and MSA patients. Equal amounts of protein (10 μg) were loaded in each lane of 15% polyacrylamide gels and proteins were resolved by SDS-PAGE and analyzed by immunoblotting. The HS and SDS/U fractions from the pons and cerebellum of 3 control or MSA patients were analyzed with 94-3A10 (top panels), 5H12 (middle panels) and 4F7 (bottom panels) antibodies, and distinct bands can be seen near ~ 17 kDa (full-length αSyn) in every HS fraction, while only in confirmed cases of MSA in the SDS/U fractions. Additional bands of sizes varying between ~ 30 and ~ 200 kDa can also be seen prominently in the SDS/U fractions of confirmed MSA and not in the controls. Antibody 4F7 detected bands for these same fractions in the pons at a molecular weight lower than 17 kDa. The relative mobility of molecular mass markers is indicated on the left of each blot. Arrows point to full-length αSyn and asterisk indicates carboxy-truncated αSyn

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