Fig. 3From: Robust α-synuclein pathology in select brainstem neuronal populations is a potential instigator of multiple system atrophyCharacterization of neuronal inclusions in the pontine nuclei of MSA patients. a Double staining of the pons of an MSA patient with anti-MAP2 (red) and anti-αSyn 5H12 (brown) antibodies. b Double staining of the pons of an MSA patient with anti-NFL (red) and anti-αSyn 5H12 (brown) antibodies. c IHC with anti-ubiquitin antibody Ubi-1. d IHC with anti-p62 antibody 5G3. In c and d, the tissue sections were also counterstained with hematoxylin. Arrows depict neuronal cell bodies within the pontine nuclei and asterisks highlight GCIs. Scale bar = 60 μm in a, b and 120 μm in c, dBack to article page