Fig. 5

Mutant HTT is localized to the electron-lucent areas within the lumen of the organelles. a–f Immunogold-labelled S830 and mEM48 antibodies were imaged on EM sections from 12-week-old R6/2 mice a, c–f shows organelles labelled with S830 10 nm gold and b with EM48 20 nm gold. d, f Enlarged views of the boxed areas in c and e, respectively, showing that the gold particles are localized to electron-lucent areas inside the organelles. g Percentage of organelles that contained gold particles after immunogold labelling. Three independent experiments were performed for S830 and two for mEM48, with approximately 15 micrographs being imaged for each experiment. h Relative numbers of gold particles inside the recruitment foci compared to those in the background areas of the same QUANTIFOIL carbon hole. Statistical analysis was by Student’s t-test. Data represented by mean ± S.D. **p < 0.01; ***p < 0.001. Scale bars: 100 nm. -1°Ab = without primary antibody