Fig. 3

Recruitment of exon 1 HTT proteins to brain sections from HD mouse models is disease-stage and polyQ-length dependent. a Representative confocal microscopy images for recruitment of HTT-exon1-43Q to the hippocampus (CA1) of R6/2 and wild-type mice at 4, 8 and 12 weeks of age. Nuclei were counterstained with Hoechst. b Recruitment of HTT-exon1-15Q, HTT-exon1-23Q and HTT-exon1-43Q, as well as HTT-exon1-43Q with an N-terminal 17 amino acid truncation (ΔN17-exon1-43Q) to 6-month-old zQ175, and 12-week-old R6/2, brain sections. Nuclei were counterstained with Hoechst. c Quantification of fluorescence intensities of HTT-exon1-43Q treatment for R6/2 and wild-type mice at 4, 8 and 12 weeks of age. d, e Quantifications of fluorescence intensities of d zQ175 and e R6/2 sections treated with exon 1 HTT proteins with different polyQ lengths. The fluorescence intensities were normalized to the wild-type control. Statistical analysis was by two-way ANOVA. Data represented by mean ± S.D., two sets of 15–25 images for three replicates per experiment. *p < 0.05; **p < 0.01; ***p < 0.001, ns = not significant. Scale bars: 20 μm. WT = wild-type