Fig. 7

G₄C₂ repeat RNAs rarely co-localize with cytoplasmic G3BP1 or nuclear TDP-43 during stress. a R-HCR-ICC of C9orf72 patient derived fibroblasts treated 1 h with H₂O or SA. G3BP1 = stress granule marker. Higher magnification images of the boxed areas are shown on the right. White arrows indicate co-localization of G₄C₂ repeat and G3BP1. b Quantification of cytoplasmic G₄C₂ repeat foci co-localization with G3BP1 as a fraction of all cytoplasmic G₄C₂ repeats. c R-HCR-ICC of C9orf72 patient derived fibroblasts treated with H₂O or SA (1 h, 2 h). TDP-43 = nuclear body marker. White arrows indicate co-localization of G₄C₂ repeat and TDP-43. d Quantification of nuclear G₄C₂ repeat foci colocalization with TDP-43 as a fraction of all observed nuclear G₄C₂ repeat foci. % shown as proportion of co-localization for each condition ± SEM. Statistics: Fisher’s exact test (b) and Chi-square test (d). **p < 0.01, ***p < 0.001. ns: not significant. Scale bar = 10 μm in (a) and (c) except for zoomed images (2.5 μm)