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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Enhanced detection of expanded repeat mRNA foci with hybridization chain reaction

Fig. 5

G4C2 repeat RNAs redistribute in the nucleus during stress in C9orf72 ALS-FTD patient fibroblasts. a R-HCR of C9orf72 ALS-FTD patient fibroblasts treated with H2O (vehicle) or sodium arsenite (SA) for the indicated times. b) Quantification of cells with detectable G4C2 repeat RNA foci in total cells. The dot shapes represent different C9orf72 patient fibroblast lines. N > 150 for each line for vehicle, SA 1.0 h, and SA 2.0 h. c Distribution of G4C2 repeat RNA foci with or without SA treatment ± 95%CI. N = 400 for vehicle, 679 for SA 1.0 h, and 775 for SA 2.0 h. Statistics: matched one-way ANOVA and paired t test (b), and Chi-square test (c). *p < 0.05, ***p < 0.001, ****p < 0.0001. ns not significant. Scale bar = 10 μm in a

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