Fig. 2
From: Enhanced detection of expanded repeat mRNA foci with hybridization chain reaction
R-HCR probe signal intensity is repeat length dependent for G4C2 and CGG repeats. a R-HCR on MEFs transfected with G4C2 repeat constructs with increasing repeat sizes. b Quantification of cells with detectable G4C2 repeat RNA foci in total cells. (G4C2)3: N = 313; (G4C2)35 N = 575; (G4C2)70 N = 514. c Quantification of R-HCR signal intensity in MEFs transfected with (G4C2)n-NL-3xF reporters with indicated repeat length. (G4C2)35: N = 175; (G4C2)70: N = 110. d R-HCR on MEFs transfected with CGG repeat constructs with increasing repeat sizes. e Quantification of Flag + and CGG RNA + cells expressed as proportion of total transfected cells. f Quantification of R-HCR signal intensity in MEFs transfected with 2(CGG)n-NL-3xF reporters with indicated repeat length. e-fN ≥ 35/condition. Error bar indicates 95% CI. Statistics: Chi-square test for b, c, e and f. Fisher’s exact test for b, **p < 0.01, ****p < 0.0001, ns: not significant. Scale bar = 50 μm in a and d