Fig. 3
Decreased B7-H3 expression in human GBM cells impairs tumor growth in vivo. a Representative images of a western blot analysis of stemness markers, Nestin or SOX2 as well as 4IgB7-H3 and 2IgB7-H3 in U87MG, GB138, T08 or T018 human GBM cells cultured as spheroids ( +) or in adherent conditions (−). b Graphs showing the weight of mice over time (days). Nude mice were grafted with U87MG human GBM cells transduced with shNT OEctrl (N = 9; blue line), shB7-H3 OEctrl (N = 9, green line) or shB7-H3 OE2Ig (N = 9, red line). c Left: Coronal sections of representative mouse brains grafted with cells described in (b). Sections are at the level of the lateral ventricles and are stained with Hematoxylin and Eosin. Right: Representative images of B7-H3 (Grey) in tumors (GFP-positive U87MG cells; green). Nuclei were counterstained with DAPI (blue). Scale bar = 50 µm. d Quantification of the tumor volumes (in µm3) in mice grafted as described in (b). Graphs are given with the mean ± SD, **p < 0.01 (ANOVA1)