Fig. 4

Profound myelin deficit in 8- and 16-week old MBP29-hα-syn (MBP29). a Graphical overview illustrating regions of interest within the mouse cerebellum including the superior (scp), middle (mcp), and inferior cerebellar peduncle (icp), as well as the pyramidal tract (py) according to [35]; coordinates: Interaural: − 2.00 mm, Bregma: − 5.79 mm. b, c Western Blot for myelin basic protein (MBP) and proteolipid protein (PLP). Quantification of MBP and PLP relative to the housekeeper β-actin (β-act) in 8- (b) and 16-week-old (c) MBP29-hα-syn compared to non-transgenic (ntg) mice (n = 5). d, e Heidenhain Woelcke staining for myelin. Quantification measured by optical density (OD) in the region of interest normalized to the molecular layer within the cerebellar cortex (cbx) in 8- (d) and 16-week-old (e) MBP29-hα-syn compared to ntg mice (n = 5–6); scale bar: 20 µm. Data represent mean + standard deviation. Statistical analyses were performed using Mann–Whitney test; *p ≤ 0.05, **p ≤ 0.01