Fig. 7

Neurodegeneration in the cortex, CA3/4 pyramidal cell layer and hilus of the dentate gyrus of mice with and without spontaneous seizures. a–c Quantitative analysis of neuronal cell loss in the cortex (a), CA3/4 pyramidal cell layer (b), and hilar interneurons (c) in control mice (n = 10) and in PTE (n = 11) and No-SRS (n = 9) mice. The mean and SEM of control animals are shown by continuous and dotted black lines, respectively. Data are the mean ± SEM and the single values. *p < 0.05, **p < 0.01 vs corresponding hemisphere in controls by one way ANOVA followed by Tukey’s post hoc multicomparison test; a #p < 0.05 vs PTE of the same hemisphere by two tailed unpaired t-test; b, c #p < 0.05 vs PTE by one-way ANOVA followed by Tukey’s post hoc multicomparison test. a1–a3, c1–c3 Photomicrographs depicting representative Nissl-stained contralateral cortices (a1–a3), and hilar interneurons (c1–c3) in the experimental groups. Scale bar: 150 µm