Fig. 1

Glioma-associated macrophages induce EC activation. a Schematic representation of experimental design. b Human umbilical vein cells (HUVEC) were incubated with media alone (EBM) or conditioned medium (CM) from RAW264.7 macrophages stimulated with normal human astrocytes (Mφ-NHA) or GBM cell lines U87, U118, U251, A172 (Mφ-U87, Mφ-U118, Mφ-U251, Mφ-A172) (upper panel) and normal human neural stem cells (Mφ-NSC) or glioma stem cell lines GSC28, GSC267 (Mφ-GSC28, Mφ-GSC267) (lower panel). mRNA was extracted and analyzed by an RT-PCR angiogenesis array. Results were normalized to RPL30 and ACTB. The relative quantity (RQ) of significantly altered genes (> twofold relative to Mφ-NHA or Mφ-NSC) are shown. n = 3, mean ± max/min, 95% confidence interval. *p < 0.05, **p < 0.01, ***p < 0.001 C. HUVEC were treated as described in (b), lysed, and immunoblotted with the indicated antibodies