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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Oral administration of repurposed drug targeting Cyp46A1 increases survival times of prion infected mice

Fig. 5

The effect of EFV treatment on PrPC level and lipid raft association in neuronal cells. a Non-infected CAD5 cell were treated with EFV (5 µM, 10 µM, 20 µM) or vehicle and PrPC in cell lysates was detected by immunoblot using mAb 4H11. β-Actin was used as a loading control. The data are indicated as the mean ± SEM for n = 3 per group, and the number of independent experiments = 3, each performed in triplicate. Significance = no significant (ns) differences b, c N2a-WT cells treated with EFV (+ EFV) or not (-EFV) were lysed in cold Triton-X 100 and subjected to flotation density gradients. Fractions 1–10 were collected from the top to the bottom of the gradient and analyzed by immunoblot using anti-PrP antibody 4H11 and anti-flotillin-1 as a lipid raft marker. b Representative immunoblot result of PrP in the fractions of cells treated with EFV. c Representative immunoblot result of PrP in the fractions of cells without EFV treatment

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