Repetitive mild traumatic brain injury in mice triggers a slowly developing cascade of long-term and persistent behavioral deficits and pathological changes

Table 2 Experimental timeline

Group #	Tests	Sample size (n) for shams and rmTBI mice at each time point	Time points
I	mTBI kinematics	4 control
II	Western Blot	6 sham (48 h after the last sham procedure) and 6 rmTBI	8 h, 48 h, 1 w and 20 w
IIa	Western blot (Naïves and Shams)	4 naïve, 5 sham (8 h after the last sham procedure) and 5 shams (48 h after the last sham procedure)	8 h, 48 h
III	DTI and MRS/ IHC/ Metabolomics	12 control and 12 rmTBI for MRS, DTI and metabolomics; 6 control and 6 rmTBI for IHC;	48 h, 1 w, 4 w and 10 w
IV	5-CSRTT	8 sham, 10 rmTBI	2 w, 6 w and 10 w
IV	EM	6 sham, 6 rmTBI	20 w
V	PVD	14 sham, 15 rmTBI	2 w, 6 w and 10 w
V	MWM	13 sham, 15 rmTBI	16 w

ISSN: 2051-5960