Table 1 Primary antibodies
Primary antibody | Host | IHC | Western Blot | Company | |
|---|---|---|---|---|---|
Dilution | Size (KD) | Dilution | |||
GFAP | Mouse | 1:500 | 51 | 1:2000 | MilliporeSigma |
Iba1 | Rabbit | 1:200 | 18 | 1:1000 | Abcam |
IL-6 | Rabbit | / | 24 | 1:1000 | Cell Signaling |
TNFα | Goat | / | 25 | 1:1000 | R&D systems |
APP | Rabbit | 1:500 | 100 | 1:1000 | Invitrogen |
Synaptophysin | Rabbit | / | 38 | 1:1000 | Cell Signaling |
PSD-95 | Rabbit | 1:100 | 95 | 1:1000 | Invitrogen |
Gephyrin | Mouse | 1:100 | 93 | 1:500 | Santa Cruz |
Beta-actin | Mouse | / | 42 | 1:5000 | Sigma |
AT8 | Mouse | 1:100 | / | / | Thermofisher |