Fig. 2

Diffuse axonal injury in corpus callosum (CC) after rmTBI. a H&E staining indicated the corpus callosum area. While background silver-staining of nuclei was visible in all sections, silver-stained fibers were not observed in the sections from control mice (b, c). Mice after a single mTBI (d) showed very few, if any, silver-stained fibers. In sections from rmTBI mice silver-stained fibers with the punctate beading typical of DAI were easily detected at 48 h, 1w, 4w and 10w after injury (e–h). Scale bar = 50 μm. Insets are high magnifications of the red-boxed areas of the panel. Inset image shows prominent silver-stained “cork-screw” shaped axons (arrows). Scale bar in insets in c-f = 20 μm