Fig. 7

TBI-induced synaptic overgrowth in the bilateral hippocampal CA1 is prevented by SCF + G-CSF treatment in the chronic phase of severe TBI. a Representative single layer of z-stack images shows PSD-95 and Gephyrin immunopositive synapses in the contralateral hippocampal CA1. b, c Quantification data show the volumes of PSD-95 (b) and Gephyrin (c) immunopositive synapses in the contralateral hippocampal CA1. d Representative single layer of z-stack images shows PSD-95 and Gephyrin immunopositive synapses in the ipsilateral hippocampal CA1. e, f Quantification data show the volumes of PSD-95 (e) and Gephyrin (f) immunopositive synapses in the ipsilateral hippocampal CA1. The red boxes in panels b, c, e and f indicate the imaging areas in the bilateral hippocampal CA1 regions for data analysis. One-way ANOVA followed by Fisher’s LSD test. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Sham: n = 3, TBI-vehicle: n = 4, TBI-SCF + G-CSF-single treatment: n = 5, TBI-SCF + G-CSF-repeated treatment: n = 5