Fig. 7
Inhibitory synaptic density is preserved in the thalamus of aged GrnR493X/R493X mice. a, b Representative hippocampal/thalamic tilescans co-stained for C1qa and Vgat in 18 month old Grn+/+ and GrnR493X/R493X mice, the dashed outline depicts area quantified in (c) (scale bar, 500 µm). A’, B’, C1qa-Vgat tilescan inset immunofluorescence images (from a, b) of thalamic VPM/VPL region in 18 month old Grn+/+ and GrnR493X/R493X brain sections (scale bar, 20 µm). c Quantification of thalamic Vgat-positive synaptic area within hippocampal/thalamic tilescans normalized to thalamic area (white dashed outline). Vgat-positive area (d), the number of Vgat-positive synaptic puncta (e), and the proportion of Vgat-positive synaptic area that is positive for C1qa (f) was quantified in high-resolution C1qa-Vgat VPM/VPL images (a’, b’). n = 10 mice were used per sex/genotype (except male GrnR493X/R493X n = 8); values are shown as mean ± SEM; ns not significant, ***p < 0.0001, Student’s t-test