From: Chronically altered NMDAR signaling in epilepsy mediates comorbid depression
Marker | Saline vs Sham (t-test) | Saline vs NI vs Flxc vs Flxc + NI | |||||
---|---|---|---|---|---|---|---|
Significant interaction? | Simple effect analysis following significant interaction: | If interaction non-significant: | Pairwise t-test with Bonferroni adjustment | ||||
Saline vs NI | Flxc vs Flxc + NI | Significant main effect for Flxc? | Significant main effect for NI? | ||||
nNOS | – | Yes; F(1,12) = 7.6, p = .0174, \(\omega_{{}}^{2}\) = .23 | Saline: mean = 1, SD = .11 / NI: mean = .97, SD = .13; d = .21, p = .78 | Flxc: mean = .75, SD = .24 / Flxc + NI: mean = 1.32, SD = .34; d = 1.96, p = .004 | – | – | *Flxc + NI significantly different from Flxc (p = .02) |
ERK1 | – | – | – | – | – | Close; F(1,12) = 4.3, p = .0602, \(\omega_{{}}^{2}\) = .2 | – |
pERK1 | – | – | – | – | – | – | – |
pERK1/ERK1 | Close, p = .123, d = 1.41 | Yes; F(1,12) = 12.48, p = .0041, \(\omega_{{}}^{2}\) = .42 | Saline: mean = 1, SD = .36 / NI: mean = .98, SD = .17; d = .06, p = .94 | Flxc: mean = .85, SD = .1 / Flxc + NI: mean = 1.6, SD = .18; d = 5.1, p = .0004 | – | – | *Flxc + NI significantly different from Saline (p = .013), NI (p = .011), and Flxc (p = .002) |
ERK2 | Yes; p = .043, d = 2.01 | – | – | – | Yes; F(1,12) = 13.83, p = .0029, \(\omega_{{}}^{2}\) = .42 | – | Flxc + NI significantly different from NI (p = .02) and Saline (p = .02) |
pERK2 | Yes; p = .017, d = 2.63 | – | – | – | Close; F(1,12) = 4.65, p = .052, \(\omega_{{}}^{2}\) = .14 | Yes; F(1,12) = 6.93, p = .0219, \(\omega_{{}}^{2}\) = .23 | Flxc + NI significantly different from Saline (p = 0.03) |
pERK2/ERK2 | – | – | – | – | – | – | – |
ERK1/2 | – | – | – | – | Close; F(1,12) = 4.28, p = .0608, \(\omega_{{}}^{2}\) = .17 | – | – |
pERK1/2/ERK1/2 | – | Yes; F(1,12) = 8.77, p = .0119, \(\omega_{{}}^{2}\) = .2 | Saline: mean = 1, SD = .1 / NI: mean = 1.06, SD = .17; d = .48, p = .7 | Flxc: mean = .9, SD = .38 / Flxc + NI: mean = 1.61, SD = .1; d = 2.51, p = .0006 | – | – | *Flxc + NI significantly different from Saline (p = .011), NI (p = .023), and Flxc (p = .004) |
ELK1 | – | – | – | – | – | Yes; F(1,12) = 8.2, p = .0143, \(\omega_{{}}^{2}\) = .3 | Difference between Flxc and NI close to significant (p = .05) |
pELK1 | Yes; p = .028, d = 2.03 | Yes; F(1,12) = 10.87, p = .0006, \(\omega_{{}}^{2}\) = .15 | Saline: mean = 1, SD = .45 / NI: mean = 1.34, SD = .62; d = .62, p = .27 | Flxc: mean = 1.19, SD = .19 / Flxc + NI: mean = 2.88, SD = .24; d = 7.92, p < .0001 | – | – | *Flxc + NI significantly different from Saline (p < .001), NI (p = .001), and Flxc (p < .001) |
pELK1/ELK1 | Close; p = .095, d = 1.40 | Yes; F(1,12) = 6.55, p = .025, \(\omega_{{}}^{2}\) = .11 | Saline: mean = 1, SD = .4 / NI: mean = .97, SD = .48; d = .83, p = .11 | Flxc: mean = 1.05, SD = .49 / Flxc + NI: mean = 2.55, SD = .13; d = 4.22, p = .0024 | – | – | *Flxc + NI significantly different from Saline (p < .001), NI (p < .001), and Flxc (p = .01) |
CREB | – | Yes; F(1,12) = 5.36, p = .0392, \(\omega_{{}}^{2}\) = .09 | Saline: mean = 1, SD = .37 / NI: mean = 1.22, SD = .17; d = .87, p = .45 | Flxc: mean = 1.22, SD = .49 / Flxc + NI: mean = 2.35, SD = .48; d = 2.31, p = .0016 | – | – | *Flxc + NI significantly different from Saline (p = .002), NI (p = .009), and Flxc (p = .01) |
pCREB | Yes; p = .012, d = 2.91 | Yes; F(1,12) = 5.81, p = .0328, \(\omega_{{}}^{2}\) = .12 | Saline: mean = 1, SD = .25 / NI: mean = 1.12, SD = .25; d = .87, p = .508 | Flxc: mean = 1.07, SD = .26 / Flxc + NI: mean = 1.92, SD = .38; d = 2.31, p = .0015 | – | – | *Flxc + NI significantly different from Saline (p = .005), NI (p = .016), and Flxc (p = .009) |
pCREB/CREB | – | – | – | – | – | – | – |
BDNF | – | Yes; F(1,12) = 13.15, p = .0035, \(\omega_{{}}^{2}\) = .14 | Saline: mean = 1, SD = .6 / NI: mean = 1.35, SD = .57; d = .55, p = .809 | Flxc: mean = .92, SD = .46 / Flxc + NI: mean = 2.47, SD = .27; d = 4.06, p = .0002 | – | – | *Flxc + NI significantly different from Saline (p = .008), NI (p = .013), and Flxc (p = .001) |
cFOS | – | Yes; F(1,12) = 37.04, p < .0001, \(\omega_{{}}^{2}\) = .3 | Saline: mean = 1, SD = 0 / NI: mean = .95, SD = .17; d = .87, p = .69 | Flxc: mean = 1.02, SD = .22 / Flxc + NI: mean = 1.97, SD = .15; d = 2.31, p < .0001 | – | – | *Flxc + NI significantly different from Saline (p < .001), NI (p < .001), and Flxc (p < .001) |