# Table 2 The statistically significant (and close to significant) differences observed between the drug combinations in their effect on molecular markers

Marker Saline vs Sham (t-test) Saline vs NI vs Flxc vs Flxc + NI
Significant interaction? Simple effect analysis following significant interaction: If interaction non-significant: Pairwise t-test with Bonferroni adjustment
Saline vs NI Flxc vs Flxc + NI Significant main effect for Flxc? Significant main effect for NI?
nNOS Yes; F(1,12) = 7.6, p = .0174, $$\omega_{{}}^{2}$$ = .23 Saline: mean = 1, SD = .11 / NI: mean = .97, SD = .13; d = .21, p = .78 Flxc: mean = .75, SD = .24 / Flxc + NI: mean = 1.32, SD = .34; d = 1.96, p = .004 *Flxc + NI significantly different from Flxc (p = .02)
ERK1 Close; F(1,12) = 4.3, p = .0602, $$\omega_{{}}^{2}$$ = .2
pERK1
pERK1/ERK1 Close, p = .123, d = 1.41 Yes; F(1,12) = 12.48, p = .0041, $$\omega_{{}}^{2}$$ = .42 Saline: mean = 1, SD = .36 / NI: mean = .98, SD = .17; d = .06, p = .94 Flxc: mean = .85, SD = .1 / Flxc + NI: mean = 1.6, SD = .18; d = 5.1, p = .0004 *Flxc + NI significantly different from Saline (p = .013), NI (p = .011), and Flxc (p = .002)
ERK2 Yes; p = .043, d = 2.01 Yes; F(1,12) = 13.83, p = .0029, $$\omega_{{}}^{2}$$ = .42 Flxc + NI significantly different from NI (p = .02) and Saline (p = .02)
pERK2 Yes; p = .017, d = 2.63 Close; F(1,12) = 4.65, p = .052, $$\omega_{{}}^{2}$$ = .14 Yes; F(1,12) = 6.93, p = .0219, $$\omega_{{}}^{2}$$ = .23 Flxc + NI significantly different from Saline (p = 0.03)
pERK2/ERK2
ERK1/2 Close; F(1,12) = 4.28, p = .0608, $$\omega_{{}}^{2}$$ = .17
pERK1/2/ERK1/2 Yes; F(1,12) = 8.77, p = .0119, $$\omega_{{}}^{2}$$ = .2 Saline: mean = 1, SD = .1 / NI: mean = 1.06, SD = .17; d = .48, p = .7 Flxc: mean = .9, SD = .38 / Flxc + NI: mean = 1.61, SD = .1; d = 2.51, p = .0006 *Flxc + NI significantly different from Saline (p = .011), NI (p = .023), and Flxc (p = .004)
ELK1 Yes; F(1,12) = 8.2, p = .0143, $$\omega_{{}}^{2}$$ = .3 Difference between Flxc and NI close to significant (p = .05)
pELK1 Yes; p = .028, d = 2.03 Yes; F(1,12) = 10.87, p = .0006, $$\omega_{{}}^{2}$$ = .15 Saline: mean = 1, SD = .45 / NI: mean = 1.34, SD = .62; d = .62, p = .27 Flxc: mean = 1.19, SD = .19 / Flxc + NI: mean = 2.88, SD = .24; d = 7.92, p < .0001 *Flxc + NI significantly different from Saline (p < .001), NI (p = .001), and Flxc (p < .001)
pELK1/ELK1 Close; p = .095, d = 1.40 Yes; F(1,12) = 6.55, p = .025, $$\omega_{{}}^{2}$$ = .11 Saline: mean = 1, SD = .4 / NI: mean = .97, SD = .48; d = .83, p = .11 Flxc: mean = 1.05, SD = .49 / Flxc + NI: mean = 2.55, SD = .13; d = 4.22, p = .0024 *Flxc + NI significantly different from Saline (p < .001), NI (p < .001), and Flxc (p = .01)
CREB Yes; F(1,12) = 5.36, p = .0392, $$\omega_{{}}^{2}$$ = .09 Saline: mean = 1, SD = .37 / NI: mean = 1.22, SD = .17; d = .87, p = .45 Flxc: mean = 1.22, SD = .49 / Flxc + NI: mean = 2.35, SD = .48; d = 2.31, p = .0016 *Flxc + NI significantly different from Saline (p = .002), NI (p = .009), and Flxc (p = .01)
pCREB Yes; p = .012, d = 2.91 Yes; F(1,12) = 5.81, p = .0328, $$\omega_{{}}^{2}$$ = .12 Saline: mean = 1, SD = .25 / NI: mean = 1.12, SD = .25; d = .87, p = .508 Flxc: mean = 1.07, SD = .26 / Flxc + NI: mean = 1.92, SD = .38; d = 2.31, p = .0015 *Flxc + NI significantly different from Saline (p = .005), NI (p = .016), and Flxc (p = .009)
pCREB/CREB
BDNF Yes; F(1,12) = 13.15, p = .0035, $$\omega_{{}}^{2}$$ = .14 Saline: mean = 1, SD = .6 / NI: mean = 1.35, SD = .57; d = .55, p = .809 Flxc: mean = .92, SD = .46 / Flxc + NI: mean = 2.47, SD = .27; d = 4.06, p = .0002 *Flxc + NI significantly different from Saline (p = .008), NI (p = .013), and Flxc (p = .001)
cFOS Yes; F(1,12) = 37.04, p < .0001, $$\omega_{{}}^{2}$$ = .3 Saline: mean = 1, SD = 0 / NI: mean = .95, SD = .17; d = .87, p = .69 Flxc: mean = 1.02, SD = .22 / Flxc + NI: mean = 1.97, SD = .15; d = 2.31, p < .0001 *Flxc + NI significantly different from Saline (p < .001), NI (p < .001), and Flxc (p < .001)
1. *While performing pairwise post hoc comparisons in the presence of a significant interaction is not strictly correct from a statistical perspective, we have used such tests to give us a better understanding of the changes from the Saline group to the Flxc + NI group