Fig. 4

Nuclear lamina invaginations are not a pathological feature in nuclei isolated from C9orf72 ALS/FTD motor cortex. a Maximum intensity projections from confocal imaging of Lamin B1 in nuclei isolated from control and C9orf72 postmortem motor cortex tissue. Genotype as indicated on top. b, c Quantification of nuclear sphericity (b) and nuclear volume (c). n = 4 control and 4 C9orf72 cases, at least 50 NeuN + nuclei per case. Student’s t-test was used to calculate statistical significance. d Quantification of number of Lamin B1 invaginations per nucleus. n = 4 control and 4 C9orf72 iPSC lines, at least 50 NeuN + nuclei per line. Chi-square test was used to calculate statistical significance. Scale bar = 5 μm